Michel Versluis, Utwente

Title: Sonoprinting and the importance of microbubble loading for the ultrasound mediated cellular delivery of nanoparticles
Session: 10:30

Abstract

In the last years, research on ultrasound mediated drug delivery using microbubbles is vastly expanding. While some groups simply mix drugs and microbubbles (co-administration), other researchers have a major interest in the potential of drug-loaded microbubbles. However, today, little is known on the pros and cons of these two strategies. In this study we evaluated the delivery of nanoparticles (polystyrene nanospheres and mRNA-lipoplexes) to cells in vitro, in case the nanoparticles were mixed with unloaded microbubbles versus loaded onto the microbubbles. Flow cytometry experiments demonstrated that unloaded microbubbles did not enhance the cellular delivery of the nanospheres and mRNA-lipoplexes. However, upon loading the nanoparticles onto the microbubbles, their delivery to cells substantially improved. Real-time swept field confocal microscopy imaging of the microbubbles and cells during ultrasound radiation revealed that nanoparticle-loaded microbubbles directly deposited the nanoparticles in patches onto the cell membrane, a process that we termed 'sonoprinting'. This phenomenon resulted in the delivery of large amounts of nanoparticles to the cells and is suggested to be different from the creation of cell membrane pores and enhanced endocytosis. Biomaterials 83 294-307(2016).

Bio

BSc Physics 1985, BSc Astronomy 1985, MSc Physics and Astrophysics 1988, PhD Nijmegen 1992) joined, University of Twente in 1999. Full Professor  Physical and Medical Acoustics in the Physics of Fluids group with research interests in micro- and nanotechnology industry.

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