Gerrit Sitters, Lumicks

Title: Acoustic Force Spectroscopy: a novel technology to perform highly parallel single molecule measurements
Session: 15:00


Force-spectroscopy has become an indispensable tool to unravel the structural and mechanochemical properties of biomolecules. Acoustic Force Spectroscopy (AFS) is a new acoustic manipulation method, which consists of a resonator integrated into a micro-fabricated fluidic chip. An acoustical pressure gradient is created homogeneously throughout the sample enabling to exert forces on bimolecular tethered microspheres. By changing the amplitude of the driving voltage the pressure gradient can be altered, allowing sensitive control of the force applied to the biomolecules. This approach allows exerting acoustic forces from sub-pN to hundreds of pN applied to thousands of biomolecules in parallel, with sub-millisecond response time and inherent stability. Well controlled acoustic forces can also be applied to other (biological) systems such as cells, lipid-membranes or hydrogels. Here we present our efforts in validating this technology by developing a stand-alone commercial-grade instrument that allows ease of operation.


to be announced

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